cfDNA reference material

  cfDNA reference material cfDNA reference material Buffer-based or plasma-based controls are produced by mixing single or multiplexed mutant gene fragments with a native, whole-genome cfDNA background (Figure B). Anchor Molecular  manufactures two types of products: Mutation Quantification Control and Mutation Reference Standard. The user can quantify either the absolute copy numbers or the relative number of the mutant gene fragments. AM Multiplexed Mutation Quantification Control Function as both positive and negative controls For use in daily assays AF% determined based on average wild-type copy numbers Customized controls available upon request Price upon request This product is used as Positive and Negative Controls in daily assays. The AF% is determined based on average wildtype copy numbers. It includes all mutant genes listed in the table below. Custom products with different parameters are available upon request. Cat. No. Base Matrix (vol) Allele Frequency (%) Wildty...

  cfDNA reference material The Plasma Genomic cfDNA Extraction Control  is for the purpose of monitoring the degree of genomic DNA (gDNA) contamination in the extracted cfDNA. DNA-free plasma is spiked with either with high molecular weight gDNA molecules or 170 bp cfDNA fragments or both. cfDNA reference material Why Extraction Control? The extraction of cfDNA from patient plasma is a complicated process that causes significant variability from run to run, kit to kit, and operator to operator. Contaminated large-sized genomic DNA interferes with the mutation quantification of cfDNA, which is mainly ~170 bp in size. Some extraction kits are better in preferentially extracting the 170bp. Figure 4 shows the cfDNA extraction Kit #1 is much better in eliminating the gDNA contamination while maintaining superior extraction yield in 170 bp DNA fragments.   AM cfDNA Extraction Specificity Controls Screens high molecular weight and DNA fragment loss Four types of specificity cont...

  cfDNA reference material Build your own controls  using your high-positive samples to save time. With our DNA-depleted human plasma (DNA-free plasma) as a matrix, you can produce reference materials specific to your time-sensitive needs while retaining the natural components of the sample. cfDNA reference material Prefer to have a knowledgeable lab do the work? Send your plasma to Anchor Molecular and we’ll remove the DNA background for you. Product Name Cat. No. Size DD Plasma (DNA-Depleted Plasma) 60601001 50, 200, 500, 1000 ml DD Serum (DNA-Depleted Serum) 60601002 50, 200, 500, 1000 ml Why Is Plasma Better Than Buffer As Reference Material Matrix? A plasma matrix is a better representation of a real patient sample because the natural, interfering proteins are retained. This allows for a more realistic process in your quality system. cfDNA reference material Equal amounts of DNA fragments were spiked into TE Buffer (blue) and human plasma (red). While the 170 bp peak was ...